2017 Research Highlights
Physiological Mg2+ conditions significantly alter the inhibition of HIV-1 and HIV-2 reverse transcriptases by nucleoside and non-nucleoside inhibitors in vitro
The efficacy of RT inhibitors is dramatically affected by the level of magnesium, unscoring the need to test inhibitors using physiological conditions.
Achuthan B, Singh K & DeStefano JJ. Biochem. 56, 33-46 (2017).
PubMed PMID: 27936595
PubMedCentral PMCID: PMC5453313
Functional interplay between murine leukemia virus glycogag, Serinc5, and surface glycoprotein governs virus entry, with opposite effects on gammaretroviral and ebolavirus glycoproteins
HIVE Center collaborators examine the effects of a cellular factor, Serinc5, upon the entry of murine leukemia virus
Ahi YS, Zhang S, Thappeta Y, Denman A, Felzpour A, Gummuluru S, Reinhard B, Murlaux D, Fivash MJ & Rein A MBio 7, e01985-16 (2017)
PubMed PMID: 27879338
PubMedCentral PMCID: PMCID5120145
An experimental check of backscattering interferometry
HIVE Center researchers validate BSI, a powerful method for rapid measuring of association constants.
Baksh MM & Finn MG. Sensors Actuators B 243, 9770981 (2017)
PubMed PMID: 28529409
PubMedCentral PMCID: PMC5433263
RiboCAT: a new capillary electrophoresis data analysis tool for nucleic acid probing
A new software package streamlines the analysis of data that probes the structure of RNA.
Cantara WA, Hatterschide J, Wu W & Musier-Forsyth. RNA, 23, 240-249 (2017)
PubMed PMID: 27821510
Analysis of RNA structure using small-angle X-ray scattering
A review of best practices for using SAXS to determine the tertiary structure of RNA under physiological conditions.
Cantara WA, Olson ED & Musier-Forsyth K. Methods , 113, 46-55 (2017)
PubMed PMID: 27777026
Conservation of tRNA mimicry in the 5′-untranslated region of distinct HIV-1 subtypes
HIVE Center researchers discover that part of the HIV genome mimics the shape of cellular transfer RNA
Comandur R, Olson ED & Musier-Forsyth RNA 23, 1850-1859 (2017).
PubMed PMID: 28860303
PubMedCentral: PMCID: PMC5689005
Dissection of specific binding of HIV-1 Gag to the “packaging signal” in viral RNA
HIVE Center collaborators uncover the details of how HIV-1 specifically recognizes its genomic RNA during packaging
Comas-Garcia M, Datta SAK, Baker L, Varma R, Gudla PR & Rein A eLIFE 6, e27055 (2017)
PubMed PMID: 28726630
PubMedCentral PMCID: PMC5531834
Inference of Epistatic Effects Leading to Entrenchment and Drug Resistance in HIV-1 Protease
HIVE researchers analyze data from patients to discover how HIV gains resistance to drugs.
Flynn WF, Haldane A, Torbett BE & Levy RM Mol. Biol. Evol. 34, 1291-1306 (2017).
PubMed PMID: 28369521
PubMedCentral PMCID: PMC5435099
Resistance to pyridine-based inhibitor KF116 reveals an unexpected role of integrase in HIV-1 Gag-Pol polyprotein proteolytic processing
A surprising connection between integrase and maturation of HIV is discovered.
Hoyte AC, Jamin AB, Koneru PC, Kobe MJ, Larue RC, Fuchs JR, Engelman AN & Kvaratskhelia M. J. Biol. Chem. 292, 19814-19825 (2017).
PubMed PMID: 28972144
PubMedCentral PMCID: PMC5712621
Potts Hamiltonian models of protein co-variation, free energy landscapes, and evolutionary fitness
A powerful method for analysis of sequence and structural data is reviewed.
Levy RM, Haldane A & Flynn WF Curr. Op. Struct. Biol. 43, 55-62 (2017).
PubMed PMID: 27870991
Multidimensional SuFEx click chemistry: sequential sulfur(VI) fluoride exchange connection of diverse modules launched from an SOF4 hub.
A new family of transformations allow click chemistry connections between diverse chemical modules, including linkages between small molecules and protein sidechains.
Li S, Wu P, Moses JE & Sharpless KB. Angewandte Chemie 56, 2903-2908 (2017).
PubMed PMID: 28165188
PubMedCentral PMCID: PMC5434761
Open and closed structures reveal allostery and pliability in the HIV-1 envelope spike
Structures of HIV-1 envelope glycoprotein with receptors and antibodies are studied with cryoelectron microscopy
Ozorowski G, Pallesen J, deVal N, Lyumkis D, Cottrell CA, Torres JL, Copps J, Stanfield RL, Cupo A, Pugach P, Moore JP, Wilson IA & Ward AB Nature 547 360-363 (2017)
PubMed PMID: 28700571
PubMedCentral PMCID: PMC5538736
A combined treatment of hydration and dynamical effects for the modeling of host-guest binding thermodynamics: the SAMPL5 blinded challenge
Absolute binding free energies are computed for host-guest complexes using a new approach.
Pal RK, Haider K, Kaur D, Flynn W, Xia J, Levy RM, Taran T, Wickstrom L, Kurtzman T & Gallicchio E J. Comput. Aided Mol. Design 31, 29-44 (2017)
PubMed PMID: 27696239
PubMedCentral PMCID: PMC5477994
Multiplex single-cell visualization of nucleic acids and protein during HIV infection
HIVE researchers present a method to observe HIV DNA, RNA and proteins inside infected cells.
Puray-Chavez M, Tedbury PR, Huber AD, Ukah OB, Yapo V, Liu D, Ji J, Wolf JJ, Engelman AN & Sarafianos SG. Nature Comm 8, 1882 (2017)
PubMed PMID: 29292235
PubMedCentral PMCID: PMC5709414
Exposing HIV’s Weaknesses
Commentary on recent research into the viral restriction factor SERINC5.
Tedbury PR & Sarafianos SG (2017) J. Biol. Chem. 292, 6027-6028
PubMed PMID: 28389578
PubMedCentral PMCID: PMC5392592