Research Highlights

The central theme of the HIVE Center is to further our structural and functional knowledge of HIV assembly, maturation, reverse transcription, and integration with the human host with the goal of determining the atomic resolution structures and the dynamic mechanisms of the macromolecular complexes involved in these processes. The ultimate aim is to relate this knowledge to the evolution of drug resistance and to the improvement of drug design methodologies in the treatment of HIV infected individuals.

This page includes publications from the HIVE Center (as compiled by NIH Reporter). Links are provided to the journal publication, the abstract at Pubmed, and in many cases, a free version of the full publication at Pubmed Central. Note that for some of the most recent articles, the Pubmed Central version may be embargoed by the journal for up to a year.

HIVE Members Presentations at the 2021 Retroviral Meeting at Cold Spring Harbor

INTERACTIONS BETWEEN HIV-1 REVERSE TRANSCRIPTASE AND HUMAN APOBEC3G

Ryan L. Slack, Darja Pollpeter, Scott J. Novick, Seongmi Kim, Kamlendra Singh, Eddy Arnold, Patrick R. Griffin, Michael H. Malim, Stefan G. Sarafianos.

A NOVEL BIS(ARYL FLUOROSULFATE) COMPOUND COVALENTLY BINDS TO THE HIV-1 CAPSID PROTEIN

Mary C. Casey, Karen A. Kirby, Hua Wang, Theresa Tiefenbrunn Sample, Philip R. Tedbury, John R. Cappiello, Bruce E. Torbett, Arthur J. Olson, K Barry Sharpless, Stefan G. Sarafianos.

ADAPTATION OF CHIMERIC HIV—INSIGHTS INTO GAG-RNA INTERACTIONS THAT LEAD TO GENOME PACKAGING

Jonathan M. Rawson, Olga A. Nikolaitchik, Jennifer A. Yoo, Xayathed Somoulay, Vinay K. Pathak, Ryan L. Slack, Stefan G. Sarafianos, Wei-Shau Hu.

MULTIOMICS PROFILING OF HIV-INFECTED CELLS REVEALS UNIQUE CHROMATIN ‘SIGNATURE’ ASSOCIATED WITH ACTIVE INTEGRATED PROVIRUSES

Raven Shah, Christian M. Gallardo, Yoonhee H. Jung, Victor G. Corces, Bruce E. Torbett, Philip R. Tedbury, Stefan G. Sarafianos.

IDENTIFY HOST FACTORS INVOLVED IN GAG TRAFFICKING AND ASSEMBLY BY USING SPLIT-APEX2 SYSTEM COUPLED WITH QUANTITATIVE MS

Shentian Zhuang, Bruce Torbett

MRHAMER YIELDS HIGHLY ACCURATE SINGLE MOLECULE VIRAL SEQUENCES ENABLING ANALYSIS OF INTRA-HOST EVOLUTION

Christian M. Gallardo, Shiyi Wang, Daniel J. Montiel-Garcia, Susan J. Little, Davey M. Smith, Andrew L. Routh, Bruce E. Torbett.

SHAPE PROBING OF THE HIV-1 REVERSE TRANSCRIPTASE INITIATION COMPLEX REVEALS RNA FLEXIBILITY CHANGES ADJACENT TO THE PRIMERBINDING SITE

Chathuri Pathirage, William Cantara, Steven Tuske, Eddy Arnold, Karin Musier-Forsyth.

ROLE OF HIV-1 5’UTR RNA STRUCTURE AND CONFORMATIONAL DYNAMICS IN GENOME PACKAGING

Jonathan P. Kitzrow, Shuohui Lui, Shiqin Miao, Kevin Jamison, Dennis Bong, Michael Poirier, Karin Musier-Forsyth.

ANALYSIS OF HTLV-1 GAG INTERACTOME AND CHARACTERIZATION OF ITS CHAPERONE ACTIVITY

Yu-Ci Syu, Joshua Hatterschide, Yingke Tang, Jacob Al-Saleem, Amanda R. Panfil, Patrick L. Green, Karin Musier-Forsyth.

STRUCTURE OF HIV-1 POL POLYPROTEIN REVEALS REVERSE TRANSCRIPTASE P66/P51 HETERODIMER-LIKE CORE: IMPLICATIONS FOR PROTEASE ACTIVATION

Jerry J Harrison, Dario Oliveira Passos, Jessica Bruhn, Joseph Bauman, Lynda Tuberty, Jeffrey DeStefano, Dmitry Lyumkis, Eddy Arnold.

HIGH RESOLUTION MAPPING OF GENIC TARGETING PREFERENCES DURING HIV-1 INTEGRATION IN VITRO AND IN VIVO

Gregory J. Bedwell, Sooin Jang, Wen Li, Parmit K. Singh, Alan N. Engelman.

STRUCTURAL BASIS FOR THE EVOLUTION OF VIRAL RESISTANCE TO LONG-ACTING HIV-1 CAPSID INHIBITOR GS-6207

Stephanie M. Bester, Arun S. Annamalai, Daniel Adu-Ampratwum, Reed Haney, James R. Fuchs, Mamuka Kvaratskhelia.

PRE-MRNA SPLICING AND GENE DENSITY ARE SIGNIFICANT DETERMINANTS OF LENTIVIRAL INTEGRATION

Parmit K. Singh, Vidya Chivukula, Peter Cherepanov, Alan N. Engelman.

GFP-TAGGED HIV-1 CAPSID PROTEIN IS SELECTIVELY PACKAGED WITH VIRAL RIBONUCLEOPROTEIN COMPLEX INSIDE THE CONICAL SHELL OF INFECTIOUS VIRIONS

Ashwanth C Francis, Parmit K Singh, Jiong Shi, Anna Cereseto, Alan Engelman, Christopher R Aiken, Gregory B Melikyan

HIV-1 INTEGRATION FAVORS GENES ASSOCIATED WITH PROMOTER-PROXIMAL POL II PAUSING

Parmit K Singh, Arun Annamalai, Mamuka Kvaratkshelia, Alan N. Engelman

UNVEILING THE GENETIC FRAGILITY OF HIV-1 THROUGH DEEP LEARNING

Juan S. Rey, Wen Li, Hagan Beatson, Christian Lantz, Alexander Bryer, Alan N. Engelman, Juan R. Perilla.

HIV-1 PIC-MEDIATED INTEGRATION IS SELECTIVELY AFFECTED BY NUCLEOSOME STRUCTURE

Nicklas Sapp, Nathan Burge, Khan Cox, Muthukumar Balasubramaniam, Jui Pandhare, Min Li, Jared Lindenberger, Mamuka Kvaratskhelia, Robert Craigie, Michael Poirier, Chandravanu Dash

DDX3 AND EIF4G1 INFLUENCE HIV-1 TRAFFICKING TO NUCLEAR SPECKLES

Szu-Wei Huang, KyeongEun Lee, Hyun Jae Yu, Stephanie V. Rebensburg, Mamuka Kvaratskhelia, Vineet N. KewalRamani.

Highlights from the HIVE Center

Informational graphics to explore current themes in HIVE research.