2023 Research Highlights
B-HIVE Member Presentations at the 2023 Retroviral Meeting at Cold Spring Harbor
Quantitative full-length sequencing of the HIV-1 transcriptome in primary cell derived resting CD4+ T-cells and T-cell subsets
Christian M. Gallardo, Nadejda Beliakova-Bethell, Bruce E. Torbett.
HIV-1 uncoating in the nucleus progresses through a distinct step of small defect formation in the capsid lattice prior to terminal uncoating
Levi Gifford, Gregory B. Melikian.
Mutations outside integrase lead to high-level resistance to integrase strand transfer inhibitors
Yuta Hikichi, Jonathan Grover, Walther Mothes, Eric Freed.
Structural insights into PPP2R5A degradation by HIV-1 Vif
Yingxia Hu, Krista A. Delviks-Frankenberry, Fidel Arizarga, Chunxiang Wu, Vinay Pathak, Yong Xiong.
Lenacapavir inhibits virion maturation by blocking formation of capsid pentamers
Szu-Wei Huang, Lorenzo Briganti, Guochao Wei, Arun Annamalai, Stephanie Bester, Reed Haney, Lauren Schope, Nikoloz Shkriabai, Ashwanth Francis, Daniel Adu-Ampratwum, James Fuchs, Alan Engelman, Mamuka Kvaratskhelia.
A critical role for the C-terminal mixed-charge domain of CPSF6 in nuclear HIV incursion and integration targeting
Sooin Jang, Hyun Jae Yu, Parmit K. Singh, Gregory J. Bedwell, Zachary M. Ingram, Satya P. Singh, AidanDarian Douglas, Zandrea Ambrose, Ashwanth C. Francis, Vineet N. KewalRamani, Alan N. Engelman.
Implications for integrase functional plasticity from the structure of the HIV-1 integrase tetramer
Tao Jing, Zelin Shan, Tung Dinh, Juliet Greenwood, Min Li, Terry Zhang, Bo Zhou, Sriram Aiyer, Robert Craigie, Alan N. Engelman, Mamuka Kvaratskhelia, Dmitry Lyumkis.
Lenacapavir disrupts HIV-1 core integrity while stabilizing the capsid lattice
Chenglei Li, Ryan C. Burdick, Sanath K. Janaka, Wei-Shau Hu, Vinay K. Pathak.
Intasomes assembled with full-length HIV-1 integrase and CTD facilitate structural studies by cryo-EM and reveal the role of the integrase C-terminal tail
Min Li, Xuemin Chen, Yanxiang Cui, Robert Craigie.
HIV-1 usurps transcription start site heterogeneity of host RNA polymerase II to maximize replication fitness
Olga Nikolaitchik, Saiful Islam, Jonathan Kitzrow, Alice Duchon, Zetao Cheng, Wei Shao, Maria Nikolaitchik, Mary Kearney, Frank Maldarelli, Karin Musier-Forsyth, Vinay K. Pathak, Wei-Shau Hu.
The role of LEDGF in transcription is intertwined with its function in HIV-1 integration
Rakesh Pathak, Parmit K. Singh, Hongen N. Zhang, Seyed Z. Rabi, Sahand Hormoz, Alan N. Engelman, Henry L. Levin.
Regulation of the timing of HIV-1 uncoating by nucleocapsid protein
Ioulia Rouzina, Helena Gien, Michael Morse, Micah McCauley, Jonathan Kitzrow, Karin Musier-Forsyth, Robert Gorelick, Mark Williams.
HIV-1 preintegration complex preferentially integrates the viral DNA into nucleosomes containing trimethylated histone 3-lysine36 modification and flanking linker DNA.
Nicklas Sapp, Nathaniel Burge, Khan Cox, Prem Prakash, Muthukumar Balasubramaniam, Devin Christenson, Min Li, Jared Linderberger, Mamuka Kvaratskhelia, Jui Pandhare, Robert Craigie, Michael Poirier, Chandravanu Dash.
Identification of host dependency factors that modulate nuclear import of HIV-1 cores
Rokeya Siddiqui, Sushila Kumari, Ryan C. Burdick, Gianluca Pegoraro, Wei-Shau Hu, Vinay K. Pathak.
CPSF6 licenses HIV-1 integration into Pol II-paused genes regulated by P-TEFb and U2 snRNP
Parmit K. Singh, Arun S. Annamalai, Mamuka Kvaratskhelia, Alan N. Engelman.
Live-cell and superresolution microscopy studies of HIV-1 restriction by MX2
Dariana Torres-Rivera, Gilberto Betancor, Michael H. Malim, Gregory B. Melikyan.
Antagonism of viral glycoproteins by guanylate binding protein 5
Hana Veler, Geraldine Vilmen, Abdul Waheed, Cheng Man Lun, Eric O. Freed.
The drug-induced interface that drives HIV-1 integrase hypermultimerization and loss of function
Atomic structures reveal the mechanism of integrase condensation by allosteric HIV-1 integrase inhibitors (ALLINIs).
Singer MR, Dinh T, Levintov L, Annamalai AS, Rey JS, Briganti L, Cook NJ, Pye VE, Taylor IA, Kim K, Engelman AN, Kim B, Perilla JR, Kvaratskhelia M, Cherepanov P. mBio. 2023 Feb 28;14(1):e0356022. doi: 10.1128/mbio.03560-22.
PubMed PMID: 36744954
PubMedCentral PMCID: PMC9973045
ViReMa: a virus recombination mapper of next-generation sequencing data characterizes diverse recombinant viral nucleic acids
An improved method is presented for identifying recombination events in long read sequences of viral genomes.
Sotcheff S, Zhou Y, Yeung J, Sun Y, Johnson JE, Torbett BE, Routh AL. Gigascience. 2023 Mar 20;12:giad009. doi: 10.1093/gigascience/giad009.
PubMed PMID: 36939008
PubMed Central PMCID: PMC10025937