Bridget Carragher, Director, and Clint Potter, Co-Director, National Resource for Automated Molecular Microscopy, TSRI, will provide automated imaging techniques for solving the three-dimensional structures of Gag, Gag-Pol, and Pol complexes using cryoTEM, evaluating the monodispersity of HIV polyproteins in conjunction with crystallization, and visualizing microcrystals in the lipidic cubic phase.
David Looney MD, University of California San Diego School of Medicine, UCSD/VMRF CFAR Molecular Biology Core, has provided a clinical perspective in the TSRI HIV Program Project. He has been instrumental in compiling, annotating, and analyzing clinical data from Southern California VA Healthcare System and CFAR Network of Integrated Clinical Systems, which provides the primary support for work on pathways of resistance mutation. Within the Center, he will continue to provide new clinical data on resistance mutations to all inhibitors.
Jason Okulicz MD, MC, USAF, is on the Infectious Disease Service Staff at Lackland Air Force Base, Texas, will provide clinical data and sera/plasma/cell samples from participates in the U.S. Military HIV Natural History Study. Okulicz will participate within the Center on the antiretroviral-mediated viral co-evolution studies by providing viral samples from individuals for protease and gag sequencing. The sequence information from viral samples will be crucial for modeling the acquisition of resistance mutations.
Alan Rein, Director of the Retroviral Assembly Section, NCI-Frederick, and staff scientist Sid Datta will collaborate on studies of HIV Gag and Gag-Pol polyprotein precursors. Using a defined assembly system that he and his colleagues have developed, Rein and Datta will work with Center investigators to map specific Gag-Gag interactions by HDX and to explore Gag and Gag-Pol interactions in assembly. Their broad expertise in studying retroviral assembly will help to place the Center’s studies of HIV polyproteins in the broader context of HIV assembly and maturation.
Doug Richman MD, Director of UC San Diego Center for AIDS Research, will provide resources from the CFAR, including core services in flow cytometry, molecular biology, translation virology, bioinformatics and genomics, protein expression and proteomics, and as well as clinic investigations. Utilizing the available CFAR Cores will leverage valuable research and clinical services for all Center grant participants.
Robin Wilner, Vice President for Global Community Initiatives, IBM World Community Grid, will continue a long-standing collaboration on the FightAIDS@Home project, which provides a distributed network of over two million internet shared processors for use in docking and drug design.